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Post doc positionPosted by: University of Sao Paulo
Posted date: Mar-16-2018
Location: Sao Paulo
A postdoc position is available at the Laboratory of Arthropod Biochemistry and Immunology (University of São Paulo, Institute of Biomedical Sciences, Department of Parasitology). The main goal of the project associate with this position is the identification of Type IV Secretion System (T4SS) effectors of Rickettsia rickettsii, etiological agent of Rocky Mountain spotted fever.
A FAPESP postdoc fellowship is available. Exclusive and full time dedication is required. The selected postdoc will receive R$ 7.174,80 /month (approximately US$ 2,050.00/month) for up to 8 months.
Skills and background:
a. Candidates must have concluded the PhD no longer than 7 years ago;
b. They must be independent, enthusiastic, critic, and innovative. It is also desirable that candidates be able to interact and work with the other research group members;
c. This position requires background in Molecular Biology and Biochemistry (confirmed by scientific articles and/or PhD thesis);
d. Candidates must have experience in biochemical, molecular biology and cellular techniques, such as: cloning and expression of recombinant proteins, immunoprecipitation, cell culture, quantitative PCR, western blot, and mass spectrometry. The ability to manipulate animals of animal facilities is desirable.
Required documents [please, send documents to Prof. Andréa Cristina Fogaça (deafogusp.br) until April 05th, 2018]:
a. Curriculum vitae;
b. Interest letter, mentioning why the candidate is interested in this position;
c. Two reference letters (from researches and professors that worked with the candidate before).
Abstract of the project “Molecular characterization of the interactions among, ticks, rickettsiae, and vertebrate host”
Ticks have parasitic habit of life, feeding obligatory on blood and/or lymph of vertebrates. As a result of hematophagy, these arthropods transmit a wide variety of pathogens. Among the tick-borne rickettsial diseases, we highlight Bovine Anaplasmosis (BA) and Rocky Mountain spotted fever (RMSF). BA is a severe disease that affects cattle, being caused by Anaplasma marginale and transmitted by Rhipicephalus microplus in Brazil. Despite the veterinary and economic importance, there is no commercial vaccine for the control of either R. microplus or BA in Brazil. Similarly, there is no commercial vaccine for prevention of RMSF, the most severe rickettsial disease that affects humans. In Brazil, the etiologic agent of RMSF, Rickettsia rickettsii, is transmitted by ticks of the genus Amblyomma. This project aims to continue the studies to elucidate the molecular factors involved in the interaction between ticks and rickettsiae with the participation of a cohesive network of researchers. The data may reveal new targets for the development of vaccines for the control of both ticks as AB and RMSF. In addition, we propose to investigate the susceptibility of A. marginale to antimicrobial components, enabling a more effective control of bovine anaplasmosis in Brazil.
1. Galletti, MFBM et al. Natural blood feeding and temperature shift modulate the global transcriptional profile of Rickettsia rickettsia. PLOS ONE v.8, p.e 77388, 2013.
2. Bifano, TD et al. Knockdown of the Rhipicephalus microplus cytochrome c oxidase subunit III COXIII gene is associated with the failure of Anaplasma marginale transmission. PLoS One. 2014 May 30;9(5):e98614. doi: 10.1371/journal.pone.0098614. eCollection 2014. Erratum in: PLoS One. 2014;9(9):e106877.
3. Rosa, RD et al. Exploring the immune signalling pathway-related genes of the cattle tick Rhipicephalus microplus: from molecular characterization to transcriptional profile upon microbial challenge. Dev Comp Immunol. 2016 Jun;59:1-14. doi: 10.1016/j.dci.2015.12.018.
4. Galletti, MFBM et al. Virulence genes of Rickettsia rickettsii are differentially modulated by either temperature upshift or blood-feeding in tick midgut and salivary glands. Parasit Vectors. 2016 Jun 10;9(1):331. doi: 10.1186/s13071-016-1581-7.
5. Capelli-Peixoto et al. The transcription factor Relish controls Anaplasma marginale infection in the bovine tick Rhipicephalus microplus. Dev Comp Immunol. 2017 Apr 10;74:32-39. doi: 10.1016/j.dci.2017.04.005.
6. Martins LA et al. The distinct transcriptional response of the midgut of Amblyomma sculptum and Amblyomma aureolatum ticks to Rickettsia rickettsii correlates to their differences in susceptibility to infection. Front. Cell. Infect. Microbiol., 28 April 2017 https://doi.org/10.3389/fcimb.2017. 00129.