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PhD student position in biophysics

Posted by: University of Greifswald

Posted date: Aug-10-2017

Location: Greifswald, Germany

One PhD Position dedicated to study the influence of oxidants derived from platelets on the activation of alphaIIbbeta3 integrin is available from October 2017 at the University of Greifswald.

 

Applications are invited from highly motived students with a background in biophysics, biology, biochemistry or related fields. Successful candidates (with experience in working with proteins and cells) will work in an interdisciplinary team of international scientists at the Center of Innovation for Humoral Immune Responses in Cardiovascular Disorders (ZIK HIKE), University of Greifswald, Germany.

 

ZIK HIKE is an interdisciplinary institute and a joint project of the faculty of natural sciences and the faculty of medicine at the University of Greifswald. 

 

The project aims to study the influence of oxidants derived from platelets on the activation of αIIbβ3. For this purpose, αIIbβ3 will be reconstituted in a membrane environment (lipid bilayers) and will let to interact with exogenous oxidants and their products (e.g. hydrogen peroxide, H2O2; superoxides, O2-; nitric oxide, .NO). Protein modification (activation) as a result of oxidative damage will be investigated using a combination of biophysical techniques. Liposome preparation and αIIbβ3 incorporation will be carried out and characterized by quartz crystal microbalance (QCM) and atomic force microscopy (AFM). Förster resonance energy transfer (FRET) will be used to prove whether or not αIIbβ3 is activated. In addition, antibodies which recognize the activated αIIbβ3 will be used in an enzyme immunosorbent assay to validate protein activation by oxidants. The interaction of oxidants and their products with αIIbβ3 will be also investigated under various pH, ions and temperature conditions

  

The project will be carried out as part of the Research Training Group "Biochemical, biophysical and biomedical effects of reactive Oxygen and Nitrogen species on biological membranes" (RTG 1947) funded by the Deutsche Forschungsgemeinschaft. For Details see the Webpage of the Graduate School (www.physik.uni-greifswald.de/dfg-forschungsverb uende/biox/). Payment and benefits are based on TV-L guidlines. 

 

Applications containing a cover letter, a CV and the contact details of two Referees should be submitted to Prof. Dr. Mihaela Delcea via E-Mail: delceam(at)uni-greifswald.de

 

Job Title PhD student position in biophysics
Post Details
Email delceam(at)uni-greifswald.de
Employer's Website http://
Category
Job Discipline Job Discipline -> Other
Job Classification Job Classification -> Other
Job Type Job Type -> Part-time
Location Greifswald, Germany
Key Words PhD student, protein activation, biophysics
Start Date October 2017
Deadline