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Assistant Project Scientist, Step I-VI - Cell/Developmental Biology - Molecular & Cell Biology

Posted by: University of California Berkeley

Posted date: Apr-05-2017

Location: Berkeley

Assistant Project Scientist, Step I-VI Cell/Developmental Biology Molecular & Cell Biology

To apply: https://aprecruit.berkeley.edu/apply/JPF01325

The Rape lab in the Molecular & Cell Biology Department at UC Berkeley seeks an Assistant Project Scientist. The goals of the Rape lab are to understand ubiquitin-dependent mechanisms of human development and disease, using a combination of structural, biochemical, cell biological, and genetic approaches. We are looking of a motivated Assistant Project Scientist with an interest in tackling these questions using multidisciplinary approaches, thereby providing a molecular understanding of pediatric diseases.


Independent research: generate mouse knockout strains using CRISPR/Cas9 genome editing
Generating genome edited human embryonic stem cell lines to mimic pediatric diseases
Execute complex affinity-purification and CompPASS mass spectrometry approaches; experience in quantitative mass spectrometry approaches is required
Perform ribosome profiling experiments, including library preparation and data analysis.
Experience in biochemical reconstitution, i.e. purification of recombinant proteins from bacterial, insect cell, and mammalian source; quantitative kinetic analyses of binding and ubiquitylation reactions using fluorescence anisotropy measurements
Lab management duties will include ordering, filling reagent requests, coordinating food supplies, and stock keeping. Supervising compliances with all safety and animal regulations, radiation safety.

Minimum/Basic Qualifications Required (By the time of application): PhD degree (or related degree) in Molecular & Cell Biology (or related field) and at least four years of postdoctoral experience.

Additional Qualifications Required (by start date): The successful candidate will have experience in neuronal differentiation assays and ribosome profiling and deep sequencing analyses methods. The candidate should also have proven experience with biochemical reconstitution, CRISPR/Cas9 genome editing, and human embryonic stem cell culture.

Preferred Qualifications (by start date): Excellent communication skills and very strong publication record.

Appointment: This position reports to Professor Michael Rape. The initial appointment is for 3 months with the expectation of extension based on satisfactory performance and availability of funding. The approximate start date of this position will be July 2017.

Salary: $54,800 - $70,900, depending on qualifications. This position is at 100% time and provides mid-level benefits.

To apply: https://aprecruit.berkeley.edu/apply/JPF01325

Interested individuals should submit application documents as PDFs, which includes, an updated curriculum vitae, and names with contact information for between 1 3 individuals who have agreed to provide a reference for this specific position and a cover letter. (Letters of reference may be requested of the finalists).

Specific questions regarding the recruitment can be directed to Sabastian Belser, HR Partner, sswbelser(at)berkeley.edu.

This recruitment will remain open until filled.

All letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e., dossier service or career center), to the UC Berkeley statement of confidentiality (http://apo.berkeley.edu/evalltr.html) prior to submitting their letters.

The University of California is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age or protected veteran status. For the complete University of California nondiscrimination and affirmative action policy see: http://policy.ucop.edu/doc/4000376/NondiscrimAffirmAct. The department is interested in candidates who will contribute to diversity and equal opportunity in higher education through their work.
Job Title Assistant Project Scientist, Step I-VI - Cell/Developmental Biology - Molecular & Cell Biology
Post Details
Email sswbelser(at)berkeley.edu
Employer's Website aprecruit.berkeley.edu
Job Discipline Job Discipline -> Molecular Biology
Job Classification Job Classification -> Research Associate
Job Type Job Type -> Full-time
Location Berkeley
Key Words Molecular, Cell, Biology, Developmental, Scientist
Start Date 07/01/2017
Salary $54,800 - $70,900
Deadline May-31-2017